pge2 neutralizing antibody  (Cayman Chemical)

Journal: Stem Cell Research & Therapy

Article Title: Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages

doi: 10.1186/s13287-020-1561-x

Figure Lengend Snippet: PGE2 mediates the polarization effects of UCMSC-CM on macrophages. LPS-treated macrophages were stimulated with NS-398-CM, a mixture of UCMSC-CM with an anti-PGE2 neutralizing antibody, or NCM plus PGE2. Several cytokines derived from macrophages were detected by ELISA. a TNF-α. b IL-6. c IL-10. d VEGF. n = 6 per group. * P < 0.05, ** P < 0.01

Article Snippet: To determine the involvement of UCMSC-secreted PGE2 in UCMSC-CM-induced macrophage polarization, LPS-treated macrophages were stimulated with NS-398-CM or a mixture of UCMSC-CM with an anti-PGE2 neutralizing antibody (10 μg/mL, Cayman Chemical).

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay

Journal: Stem Cell Research & Therapy

Article Title: Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages

doi: 10.1186/s13287-020-1561-x

Figure Lengend Snippet: PGE2 plays a role in the UCMSC-CM-induced functional recovery of diabetic vascular endothelial cells via remodeling of the macrophage phenotypes. High-glucose-induced HUVECs, which were indirectly cocultured with macrophages, were treated with NS-398-CM, a mixture of UCMSC-CM with an anti-PGE2 neutralizing antibody, or NCM plus PGE2. The functions of HUVECs were determined by angiogenesis assays, migration assays, and chemotaxis assays. a Representative images of the angiogenesis assay. Scale bar 200 μm. b Quantitative analysis of the total tube length in the angiogenesis assay. c Representative images of the migration assay. Scale bar 200 μm. d Quantitative analysis of the wound area in the migration assay. e Quantitative analysis of the chemotactic cell number in the chemotaxis assay. n = 6 per group. * P < 0.05, ** P < 0.01

Article Snippet: To determine the involvement of UCMSC-secreted PGE2 in UCMSC-CM-induced macrophage polarization, LPS-treated macrophages were stimulated with NS-398-CM or a mixture of UCMSC-CM with an anti-PGE2 neutralizing antibody (10 μg/mL, Cayman Chemical).

Techniques: Functional Assay, Migration, Chemotaxis Assay, Angiogenesis Assay

Journal: Stem Cell Research & Therapy

Article Title: Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages

doi: 10.1186/s13287-020-1561-x

Figure Lengend Snippet: Schematic model of UCMSCs promoting wound healing in diabetic mice. UCMSCs accelerate wound healing and improve angiogenesis in diabetic mice via the remodeling of the macrophage phenotypes. UCMSC-secreted PGE2 plays a crucial role in improving the restoration of vascular endothelial cell dysfunction via the remodeling of macrophage phenotypes, which subsequently improve the local microenvironment of vascular endothelial cells through the secretion of IL-10 and VEGF

Article Snippet: To determine the involvement of UCMSC-secreted PGE2 in UCMSC-CM-induced macrophage polarization, LPS-treated macrophages were stimulated with NS-398-CM or a mixture of UCMSC-CM with an anti-PGE2 neutralizing antibody (10 μg/mL, Cayman Chemical).

Techniques:

Journal: The Journal of Experimental Medicine

Article Title: Ovarian cancer progression is controlled by phenotypic changes in dendritic cells

doi: 10.1084/jem.20111413

Figure Lengend Snippet: Tumor cell–derived PGE2 and TGF-β1 promote the immunosuppressive activity of DCs. (A) Quantification of PGE2, mature TGF-β1, and IL-6 in media conditioned by tumor cells from advanced (∼60 d) ovarian cancer specimens, in the presence or the absence of specific neutralizing antibodies. +BMDCs, BMDCs were incubated for 2 d in tumor-conditioned media before cytokine quantification; cRPMI, RPMI control media; TCmedia, tumor-conditioned media plus an irrelevant IgG; TCmedia+NeuAb, tumor-conditioned media plus specific neutralizing antibodies. Error bars, SEM. *, P < 0.05. (B) PD-L1 expression in splenic CD45 + CD11c + MHC-II + DCs sorted from mice carrying early (day 7) tumor lesions, cultured with control versus tumor-conditioned media, in the presence or the absence of specific neutralizing antibodies. (C) Splenic DCs (spDCs) sorted from early tumor-bearing mice were cultured for 48 h in RPMI or tumor-conditioned media (TCMed), in the presence of neutralizing anti-PGE2 or anti–TGF-β1 antibodies (NeuAb), or an irrelevant IgG. These cultured DCs were then incubated with tumor-pulsed BMDCs plus CFSE-labeled tumor-reactive T cells, obtained as in (1:1:1 ratio). Unpulsed BMDCs were added to control wells. The panel shows representative histograms of the proliferation of tumor-reactive T cells under different conditions. Representatives of two independent experiments are shown.

Article Snippet: When indicated, neutralizing antibodies against mouse PGE2 (7 μg/ml; 2B5; Cayman Chemical), TGF-β1 (5 μg/ml; 2Ar2; Abcam) or IL-6 (4 μg/ml; PeproTech) were added to the media.

Techniques: Derivative Assay, Activity Assay, Incubation, Control, Neutralizing Assay, Expressing, Cell Culture, Labeling